Identification of acis-Acting Dendritic Targeting Element in MAP2 mRNAs
نویسندگان
چکیده
منابع مشابه
Identification of a cis-acting dendritic targeting element in MAP2 mRNAs.
In neurons, a limited number of mRNAs have been identified in dendritic processes, whereas other transcripts are restricted to the cell soma. Here we have investigated the molecular mechanisms underlying extrasomatic localization of mRNAs encoding microtubule-associated protein 2 (MAP2) in primary neuronal cultures. Vectors expressing recombinant mRNAs were introduced into hippocampal and sympa...
متن کاملDendritic localization of mRNAs.
The dendritic localization of mRNAs has been proposed to underlie the structural and functional polarity of neurons, as well as certain aspects of synaptic plasticity. Even though there is no conclusive evidence that such a localization is a physiological requirement, studies of mRNA localization in relation to function in other cell types and recent experiments on synaptic plasticity suggest t...
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The mechanisms underlying the subcellular localization of neurotrophins and their receptors are poorly understood. We show that in cultured hippocampal neurons, the mRNAs for BDNF and TrkB have a somatodendritic localization, and we quantify the extent of their dendritic mRNA localization. In the dendrites the labeling covers on average the proximal 30% of the total dendritic length. On high po...
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In neurones, a limited number of mRNAs is found in dendrites, including transcripts encoding the microtubule-associated protein 2 (MAP2). Recently, we identified a cis-acting dendritic targeting element (DTE) in MAP2 mRNAs. Here we used the yeast tri-hybrid system to identify potential trans-acting RNA-binding factors of the DTE. A cDNA clone was isolated that encodes a member of a mammalian pr...
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Streak events are identified primarily on the basis of coincidence of > ν events occurring in a single chipy row of a single node within a single frame (Figure 1). The grade and fltgrade distributions of the streak events are similar to those of “good” events (Figure 2). The energy spectrum peaks at E ≈ 0.5 keV and shows weak line emission features, the strongest of which lies at 1.8 keV and is...
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ژورنال
عنوان ژورنال: The Journal of Neuroscience
سال: 1999
ISSN: 0270-6474,1529-2401
DOI: 10.1523/jneurosci.19-20-08818.1999